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enzyme catalysis
Title: enzyme catalysis
Category: Science & Technology
Details: Words: 2770 | Pages: 11.8 (approximately 235 words/page)
enzyme catalysis
Glucose-6-Phosphate Appears to be a Competitive Inhibitor of the Bovine Intestine Enzyme Alkaline Phosphatase
Abstract
This study focuses on the effects of glucose-6-phosphate (G6P) on the kinetics of the enzyme alkaline phosphatase. Because G6P is a common molecule and has a phosphoester bond, it may prevent alkaline phosphatase from hydrolyzing certain substrates. The data collected in this study seem to indicate that G6P is a competitive inhibitor of the enzyme …showed first 75 words of 2770 total…
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…showed last 75 words of 2770 total…Determining Vmax and Km
From a Lineweaver-Burk plot, the y intercept = 1/Vmax and the x-intercept = -1/Km
Without G6P: y=0.7505x+17.563
y intercept = 17.563 x intercept = -17.563/0.7505
Vmax = 1/17.563 = -23.27
= 0.0573 umole/min Km = -1/-23.27
= 0.043 mM
With G6P: y=1.5121x+15.979
y intercept = 15.979 x intercept = -15.979/1.5121
Vmax = 1/15.979 = -10.57
= 0.0626 umole/min Km = -1/-10.57
= 0.095 mM
Determining Ki
x intercept = -1/ ((1+I/Ki)*Km)
where x intercept is from graph with G6P, I = concentration of inhibitor, and Km is without G6P
-10.57= -1/((1+0.1/Ki)*0.043)
(1+0.1/Ki)*-0.454=-1
1+0.1/Ki = 2.20
0.1/Ki = 1.20
Ki = 0.083
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